Phenolic extract of Libidibia ferrea inhibits dentin endogenous enzymatic activity depending on the adhesive system strategy.

This study evaluated the influence of Libidibia ferrea (Lf) extract used as dentin pretreatment on the resin-dentin bond strength stability and dentin endogenous enzymatic activity. The phytochemical profile (PP) of the Lf extract was evaluated by liquid chromatography; particle size, polydispersity index (PdI), and zeta potential (ZP) were evaluated by dynamic light scattering. The tested groups were ER-Scotchbond Universal (SBU) in the etch-and-rinse (ER) mode; ERLf-SBU in the ER mode + Lf after etching; SE- SBU in the self-etch (SE) mode; and LfSE-Lf before SBU in the SE mode. Sticks were obtained for microtensile bond strength tests and failure mode (24 hr and 12 months). The hybrid layer was evaluated using scanning electron microscopy. The endogenous enzymatic activity of the underlying dentin was analyzed by in situ zymography with the same treatments. The PP showed the presence of quercetin (2.6% w/w). Lf particles were considered large after the analysis of the PdI. The ZP remained stable over time. The ER and ERLf groups had lower bond strength after 12 months, but SE and LfSE remained stable. The predominant failure mode was adhesive for both times. ER and ERLf had longer resin tags and a thicker hybrid layer. The ER and LfSE groups showed higher enzymatic activity than the ERLf and SE groups after 12 months. The Lf extract may contribute to inhibit the dentin endogenous enzymatic activity when associated with an adhesive system in the ER mode.

Microbial contamination of a University dental clinic in Brazil

Pathogens of the oral cavity of a patient can be transferred to the dental office surfaces by direct contact, aerosol instruments and blood or saliva. The objective of this study was to investigate the microbiological contamination presents in the stands, chairs and spittoons in the University Nilton Lins dental clinics, in Manaus, Amazonas. Samples were collected with sterile swabs and seeded in different microbiological culture media for the isolation of microorganisms collected from each room. Then, assays were carried out for identification of strains isolated from each environment, such as: Gram stain, DNA purification, Amplification of 16s rRNA genes and sequencing. All these experiments were performed in the LBS / ILMD / FIOCRUZ. It was found 40 CFU / mL in the stands, 43 on the chairs and 47 in the spittoons and it was also possible to identify microorganisms like Klebsiella pneumoniae, Shigella sonnei and Staphylococcus aureus. The greatest number of CFUs was found in Clinic 3 and it was observed that the spittoon was the dental surface with the highest number of CFUs. Some of the bacterial species isolated are opportunists, suggesting that more severe biosecurity measures must be taken in order to prevent cross-infection (AU)

Aesthetic and functional rehabilitation with Alumina: a case report / Reabilitação estética e funcional com Alumina: Relato de caso

A restauração totalmente cerâmica de dentes anteriores é um desafio na atual situação clínica onde há uma variedade de sistemas cerâmicos disponíveis. O objetivo deste estudo é apresentar um relato de caso com a confecção de duas próteses fixas unitárias metal-free em dentes anteriores. Paciente, 30 anos de idade, gênero masculino, estudante, apresentou-se à Faculdade de Odontologia da Universidade Federal do Amazonas, preocupado com a aparência dos dentes. O retratamento endodôntico dos elementos 11 e 12 foi realizado e, em seguida, foram colocados pinos de fibra de vidro. Os preparos dentários foram feitos usando a técnica da silhueta. As coroas foram cimentadas com cimento resinoso. O paciente ficou muito satisfeito com o seu novo sorriso, já que o tratamento proporcionou a reabilitação estética dos dentes anteriores

All-ceramic restoration of anterior teeth is a challenging clinical situation for which a variety of all-ceramic systems are available. The aim of this study is to present a case report of the construction of two single-unit metal-free fixed prostheses in anterior teeth. A 30-year-old male patient came to the Faculty of Dentistry, Federal University of Amazonas, concerned about his teeth. Elements 11 and 12 were endodontically retreated and fiberglass intracanal retainer posts were inserted. Dental crowns were prepared using the silhouette technique. The crowns were cemented with resin cement. The patient was very satisfied with his new smile and the aesthetic rehabilitation of the anterior teeth provided by the treatment

Reabilitação bucal com próteses conjugadas ­ relato de caso / Oral rehabilitation with conjugated prosthesis ­ case report

A conjugação entre próteses fixa e removível representa hoje um universo de possibilidades para reabilitações. Nas próteses conjugadas, os encaixes merecem um destaque especial, pois fornecem vantagens nos aspectos biológicos, mecânicos e estéticos. O objetivo do presente trabalho é apresentar, por meio de relato de caso clínico, a associação entre prótese parcial removível (PPR) e prótese parcial fixa (PPF) com o uso de encaixes extracoronários. Paciente Classe III de Kennedy superior procurou atendimento odontológico para confecção de PPR superior sem uso de grampo. No exame clínico e radiográfico verificou-se a necessidade de restauração indireta nos elementos 13 e 23. A partir dos modelos de estudo montados em articulador semiajustável (ASA), o planejamento do caso baseou-se na confecção de PPR superior associada a coroas metalocerâmicas nos caninos superiores utilizando o sistema de encaixe do tipo attachment e PPR inferior convencional. A utilização de próteses fixa e removível conjugadas com encaixes tipo attachment demonstrou ser uma solução viável estética e funcionalmente, proporcionando conforto para a paciente, com prognóstico favorável à manutenção das estruturas (AU).

The combination between fixed and removable dentures opens a range of new possibilities for rehabilitation. The combined prosthesis fittings deserve special attention because they provide advantages in biological, mechanical and aesthetics. The aim of this paper is to present, through a case report, the association between removable partial denture (RPD) and fixed partial denture (FPD) with the use of extra-coronal fittings. Patient with upper Kennedy Class III sought for dental care for making an upper RPD without staple use. Clinical and radiographic exams indicated the need for indirect restoration of elements #13 and #23. From the study models mounted on the semi-adjustable articulator (SAA), the case planning was based on the manufacture of upper RPD associated with metal-ceramic crowns on the maxillary canines using the system plug the attachment type and conventional lower RPD. The use of fixed and removable dentures combined with attachment fittings proved to be a viable solution esthetically and functionally providing comfort to the patient, with a prognosis in favor of maintaining the structures (AU).

Herbal mouthwash based on Libidibia ferrea: microbiological control, sensory characteristics, sedimentation, pH and density / Enxaguatório bucal fitoterápico à base de Libidibia ferrea: controle microbiológico, características organolépticas, sedimentação, pH e densidade

Introduction: Phytotherapy is the study of herbal medicines and their applicability to cure diseases in general, being a therapeutic method which can be used for the prevention and treatment of mouth diseases. Among the herbal studied, the Libidibia ferrea, known as jucá or ironwood, is widely used in folk medicine by presenting anti-inflammatory, analgesic, antimicrobial and antipyretic therapeutic properties. Objective: To evaluate in vitro pharmacological stability of the Libidibia ferrea extract's mouthwash (INPA - 228 022). Material and method: It was held the mouthwash microbiological control by determining the total number of microorganisms and Salmonella sp, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus; stability characteristics (color, odor, brightness and consistency), sedimentation test (centrifuge), the pH measurement (pH meter) and density evaluation (pycnometer) were analyzed. Result: The mouthwash showed to be absent from microorganisms and no changes were observed in the organoleptics and sedimentation characteristics. The average pH values were 6.21, 6.15 and 5.85 at 0, 30 and 60 days, respectively, and 1.029, 1.033 and 1.035 g/ mL density values, respectively, without interfering with the final characteristic of the formulation. Conclusion: The mouthwash presented pharmacological stability and quality conditions. .

Introdução: A Fitoterapia é o estudo de plantas medicinais e suas aplicabilidades para a cura de doenças em geral, constituindo um método terapêutico que pode ser utilizado para a prevenção e tratamento de doenças bucais. Dentre as plantas estudadas, a Libidibia ferrea, conhecida como jucá ou pau ferro, é bastante utilizada na medicina popular por apresentar propriedades terapêuticas anti-inflamatória, analgésica, antimicrobiana e antitérmica. Objetivo: Avaliar, in vitro, a estabilidade farmacológica de um enxaguatório bucal fitoterápico à base do extrato de Libidibia ferrea (228.022 - INPA). Material e método: Foi realizado o controle microbiológico do enxaguatório através da determinação do número total de microrganismos de Salmonella sp., Escherichia coli, Pseudomonas aeruginosa e Staphylococcus aureus; foram analisadas as características organolépticas (cor, odor, brilho e consistência), sedimentação (centrífuga), aferição do pH (peagâmetro) e densidade (picnômetro). Resultado: O enxaguatório mostrou-se ausente de microrganismos e não foram observadas alterações das características organolépticas e sedimentação. Os valores médios de pH foram de 6,21, 6,15 e 5,85 nos tempos de armazenamento de 0, 30 e 60 dias, respectivamente, e de densidade 1,029, 1,033 e 1,035 g/ mL, respectivamente, porém sem interferência na característica final da formulação. Conclusão: O enxaguatório à base de Libidibia ferrea apresentou condições de estabilidade e qualidade farmacológicas. .

Reabilitação bucal com prótese total imediata / Oral rehabilitation with immediate full denture

O sucesso da reabilitação protética, estética e funcional requer, na maioria dos casos, um plano de tratamento transdisciplinar. Este trabalho tem como objetivo apresentar um caso de reabilitação bucal com a confecção de prótese total imediata (PTI) na arcada superior e prótese parcial removível provisória na arcada inferior. Os resultados demonstraram que o tratamento reabilitador com PTI foi resolutivo para o caso clínico, restabelecendo estética e função sem submeter a paciente a um período de edentulismo.

In most cases the success of prosthetic, esthetic and functional rehabilitation requires a multidisciplinary treatment plan. This work aims to present an oral rehabilitation case with immediate full denture (IFD) and lower provisional removable partial denture. The results showed that the rehabilitation treatment with IFD was the solution for the clinical case, restoring aesthetics and function without submitting the patient to an edentulous period.

Conservative esthetic solution with ceramic laminates: literature review

Introduction and Objective: Modern dentistry seeks to offer increasingly esthetic treatments, aimed at patient's well-being and satisfaction. In this perspective, this study aimed to conduct a literature review on the use of ceramic laminates as conservative and esthetic solution, addressing their indications, contraindications, limitations and its importance in dentistry. Literature review: The evidence in the literature show that the ceramic laminates are used for restoring anterior teeth, reestablishing dental function and esthetic mainly due to its excellent optical property, and preserving tooth structure, as it is necessary less tooth wear to return shape, texture, color and harmony to the teeth involved. Conclusion: It was conclude that this is a treatment option with high success rate when properly indicated, planned and executed according to each case.

Implante cone morse de carga imediata com coroa CAD-CAM - relato de caso / Immediate loading Morse taper implant with CAD-CAM crown - case report

Os implantes dentários mostraram grande avanço nos últimos anos, sendo uma realidade a reabilitação bucal através da carga imediata implantossuportada. Os implantes cone morse podem ser utilizados com sucesso para todas as realidades clínicas e estão sendo cada vez mais utilizados como eleição para a reposição de dentes ausentes. Com o advento da tecnologia CAD/CAM (desenho de uma estrutura protética em um computador, seguido de sua usinagem por máquina de fresagem) houve mudanças significativas na obtenção de próteses e infraestruturas protéticas. Nessa perspectiva, o objetivo deste estudo é reportar um caso clínico utilizando-se o implante cone morse unitário com coroa CAD/CAM de carga imediata. Conclui-se que o correto planejamento, indicação e execução da terapia escolhida são fundamentais para a satisfação do paciente, podendo o implante do tipo cone morse com carga imediata ser utilizado com sucesso em casos de ausência dentária unitária posterior e que a tecnologia CAD/CAM permite a produção de coroas protéticas de alta qualidade quando as etapas de confecção são realizadas de maneira adequada.

Dental implants have shown great progress in the recent years, and oral rehabilitation with implant-supported immediate load became a reality. The Morse taper implants can be successfully utilized for all clinical conditions and are increasingly being used as an option to replace missing teeth. With the advent of CAD/CAM technology (design of a prosthetic structure on a computer, followed by a milling machine for machining) there were significant changes in obtaining prostheses and prosthetic infrastructure. In this perspective, the aim of this study was to report a clinical case using single tooth implantation with morse taper implant and CAD/CAM immediate loading crown. We conclude that the correct planning, execution and display of the selected treatment are the key to patient’s satisfaction, and immediate load morse taper implant can be used successfully in cases of single tooth absence. In addition CAD/CAM technology enables the production of high quality prosthetic crowns when manufacturing steps are properly carried out

Evaluation of the stability and antimicrobial activity of an ethanolic extract of Libidibia ferrea.

Biofilm is a dense, whitish, noncalcified aggregate of bacteria, with desquamated epithelial cells and food debris creating conditions for an imbalance of resident oral microflora and favoring the destruction of hard and soft tissues by development of caries and gingivitis. The aim of this study was to obtain and characterize an extract of Libidibia ferrea, ex Caesalpinia ferrea L. and to evaluate its feasibility for formulation as a mouthwash, according to current legislation. For this purpose, pH, sedimentation, density, and stability were evaluated, along with microbiological testing of the extract. The microbiological test was used to verify the presence of Staphylococcus aureus, Pseudomonas aeruginosa, fungi, yeasts, coliforms, and minimum inhibitory concentrations of Streptococcus mutans and Streptococcus oralis strains. Characterization, microbiological evaluation, and minimum inhibitory concentration results were tabulated and described using descriptive statistics. The L. ferrea extract showed stable characteristics, product quality, and antibacterial activity against the microorganisms tested irrespective of experimental time intervals. According to these results, it can be concluded that formulation of a mouthwash containing L. ferrea extract to control biofilm is feasible, but further studies are needed.

Prótese unitária sobre implante: uma alternativa após fracasso de tratamento endodôntico / The implant supported unitary prosthesis: an alternative after failure endodontic treatment

O plano de tratamento com próteses mudou significativamente nos últimos anos. Todos os dentes que apresentam tratamento endodôntico precisam ser analisados de maneira criteriosa para concluir se a obturação encontra-se adequada ou se o retratamento será necessário. O insucesso endodôntico geralmente é decorrente de fatores técnicos e patológicos ou influenciado por fatores sistêmicos. Os avanços na taxa de sucesso dos implantes dentários unitários tornam a prótese sobre implantes mais uma alternativa restauradora, obrigatória na opção de técnicas usadas pelo cirurgião dentista. Inúmeros fatores afetam a decisão entre o tratamento endodôntico, exodontia ou instalação de implante. Nessa perspectiva, o objetivo deste estudo foi realizar uma revisão da literatura sobre os limites do tratamento endodôntico, considerando a prótese unitária sobre implante como uma alternativa viável. A revisão de literatura mostrou que as taxas de sucesso dos implantes dentários e tratamentos endodônticos são elevadas e similares quando respeitadas as normas e os protolocos estabelecidos para cada situação; a prótese unitária sobre implante é uma alternativa importante após fracasso de tratamento endodôntico

The prosthetic treatment plan has changed significantly in recent years. All teeth that have endodontic treatment need to be examined very carefully to conclude if the root canal obturation presented is adequate or if the retreatment will be necessary. The endodontic failure is usually due to technical and pathological factors or influenced by systemic factors. Advances in the success rate of dental implants make the unit prosthesis an alternative implant restorative, required among the options of techniques used by any dentist. Numerous factors affect the decision between endodontic treatment or extraction followed by implant installation. From this perspective, the objective of this study was to make a review of literature about the limits of endodontic treatment, considering the unitary prosthesis on implants as a viable alternative. This search showed that success rates of dental implants and root canal treatments are high and similar when in compliance with the standards of fabrication and the protocols established for each situation; the unitary prosthesis on implants is an important alternative after failure of endodontic therapy